ELISA-based assay for DNA/RNA

Keith Hutchison KEITHH at MAINE.MAINE.EDU
Wed Feb 20 16:17:32 EST 1991


The sandwich method for detecting hybrids in an ELISA assay apparently
works very well as it is the basis of an assay from Hood's group
(see PNAS 87: 8923-8927 [1990]).  You would coat the wells with
streptavidin and biotinylate the RNA.  Then used digoxigenin to
tag the probe.  We haven't tried it yet, but it sounds pretty
straightforward.

Keith Hutchison



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