Anyone done PCR directly from cells

WEH%FDACFSAN at PUCC.PRINCETON.EDU WEH%FDACFSAN at PUCC.PRINCETON.EDU
Thu Jan 10 07:36:41 EST 1991


We've had good success with some bacterial cells by simply boiling for
a minute or two.  Depending on how you want to do it, a couple of
microliters of this *boilate* can be added directly to a PCR tube or you
can try precipitating with ethanol.  You can also lyse cells with
guanidine isothiocyanate and then ethanol precipitate.



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