Anyone done PCR directly from cells

[WEH%FDACFSAN at PUCC.PRINCETON.EDU]

We've had good success with some bacterial cells by simply boiling for
a minute or two.  Depending on how you want to do it, a couple of
microliters of this *boilate* can be added directly to a PCR tube or you
can try precipitating with ethanol.  You can also lyse cells with
guanidine isothiocyanate and then ethanol precipitate.