seeking bacterial strains

JMILLER%VXBIO.SPAN at STAR.STANFORD.EDU JMILLER%VXBIO.SPAN at STAR.STANFORD.EDU
Wed Jan 30 21:42:35 EST 1991


  I am trying to collect strains of bacteria which belong to
the Enterobacteraceae (for those molecular biologists out there,
E. coli has a lot of relations) for an engineering/evolutionary study. 
We plan to screen them for induced beta-galactosidase activity and 
catabolite repression, which should tell us whether they have the 
lac repressor/CAP protein system.
  Those organisms which turn out to have a lac repressor will be
used in PCR reactions to attempt to recover the actual sequences of
the repressor.
  Since the presence of the repressor/CAP system may have some relevance
to those trying to untangle the taxonomy of the Enterobacteraceae, we
are offering to test any strains you send us for these phenotypes. Since
we are molecular biologists, we don't have the expertise to interpret
our results in terms of taxonomy/plylogeny, but possibly others will
be able to use the data, and collaborations are welcome.
  What follows is a list of the type of organisms we are searching for.
We will take them from any source--lab stocks, clinical isolates, old
lyophylized cultures, etc. We already have ATCC strains for some of
these organisms, but have found that "identical" organisms can give
widely differing results in the PCR reaction.
  If interested, contact us at the following address:

Dr. Peter Markiewicz
Dept. Microbiology
421 MBI UCLA
Los Angeles, CA 90024
213-825-8924

e-mail:  JMILLER%VXBIO.SPAN at STAR.STANFORD.EDU

  We also have a parallel project to PCR out sequences for Thymidylate
synthase from major divisions of eubacteria and archebacteria. If you
are working with some of these organisms and are interested in getting
these sequences, contact us.
*************************************************************************

List of Potential Lac repressor-Positive Members of the Enterobacteraceae
any of these strains would be welcome
(note-some names may be obsolete)


Name			Lac+	ONPG+ 	b-gal	color		Reference	

Aeromonas
.sobria		d	+	?	?		5),p.1293
.caviae		d	+	?	?		5),p.1293

Alteromonas(marine)
.macleodii		+	?	?	?		7),p.1325
.espejiana		+<35!c	?	?	 	7),p.1325


Beneckea (marine,reclassified into Vibrio)
.harveyi		+	?	?	lum.61	6),p.1323
.campbellii		+	?	?	-		6),p.1323
.parahaemolytica	+	?	?	-		6),p.1323	
.alginolytica	+	?	?	-		6),p.1323
.natrigens		+	?	?	-		6),p.1323
.vulnifica		+	?	?	-		6),p.1323
.splendida		+	?	?	lum.+		6),p.1323
.pelagia		+	?	?	-		6),p.1323
.nereida		+	?	?	-		6),p.1323
.angulillarum	+	?	?	-		6),p.1323
.nigrapulchrituda	+	?	?	-		6),p.1323
.proteolytica	+	?	?	-		6),p.1323

Buttiauxella
.agrestis		100	100	?	0		1),p.48-52

Cedecca	
.lapagei		60	100	?	0		1),p.48-52
.neteri		35	100	?	0		1),p.48-52

Citrobacter	
.freundii		50	100	?	0		1), p.48-52
.diversus		35	100	?	0		1), p.48-52
.amalonaticus	50	100	?	0		1), p.48-52

Enterobacter
.aerogenes		95	100	?	0		1), p.48-52
.agglomerans	40/94	90	?	75	(groups 2-11,not assigned to Erwinia)
.amnigens		50	100	?	0		1), p.48-52	
.asburiae		?	?	?	?     (also known as one of agglomerans groups)
.cloacae		93	99	?	0		1), p.48-52
.dissolvens		?	?	?	?		8), p.338
.gergoviae		55mut	97	?	0		1), p.48-52
.intermedium	100	100	?	0		1), p.48-52
.nimipressuralis	?	?	?	?
.taylorae		?	?	?	?


Erwinia	
.amylovora		100	?	?	?		10), p. 9-11
.ananas		100	?	?	?		10), p. 9-11
.carotovora		100	?	?	?		10), p. 9-11
.chrysanthemi	68	?	?	?		10), p. 9-11
.rhapontici		100	?	?	?		10), p. 9-11
.stewartii		30	?	?	?		10), p. 9-11
.uredovora		100	?	?	?		10), p. 9-11

Escherichia-Shigella
.adenylcarboxylata (really an Erwinia?)
.hermanii		45	98	?	0		1), p.48-52

Ewingella
.americana		70	85	?	0		1), p.48-52	

Klebsiella		
.planticola		30	100	?	0		1),p.48-52
.terrigena		100	100	?	0		1),p.48-52
.trevisanae		?	?	?	?		

Kluyvera
.ascorbata		98	100	?	0		1), p.48-52
.cryocrescens	95	100	?	0		1), p.48-52

Levinea 
.amalonatica	?	?	?	?		8), p 339

Leciercia 
.adencarboxylata	?	?	?	?		9), p. 65

Leminorella
.grimontii		?	?	?	?		9), p. 65
.richardii		?	?	?	?		9), p. 65

Moellerella
.wisconsensis	100	90	?	0		1), p.48-52

Photobacterium
.leiognathi		+	?	?	lum.+		6),p.1323
.angustum		+	?	?	lum.+		6),p.1323
.phosphoreum	+	?	?	lum.+		6),p.132


Plesiomonas	
.shigelloides	d	+	?	?		Chap. 103,

Pragia 
.fontium		?	?	?	?		9), p. 65

Rhanella
.aquatilis		100	100	?	0	1), 	p.48-52

Salmonella
subgroup 3b(Arizona)	85	100	?	0	1), p.48-52

Serratia
.entophilia		?	?	?	?		
.ficaria		?	?	?	?		8), p.339
.fonticola		97	100	?	0		(Erwinia?) 8), p. 339
.grimesii		?	?	?	?		8), p.339
.marinobura		+	+	?	red		2),chap97,p1196
.odorifera		97mut	100	?	0		1), p.48-52
.plymuthica		80mut	100	?	0		1), p.48-52
.proteamaculans	?	?	?	?		9), p.65
.rubidaea		100	100	100	0		1), p.48-52

References:
1)Farmer et. al., (1985) J. Clin. Microbiology 21(1),46-76
The Prokaryotes (1981) A handbook on habits, isolation, and identification
of Bacteria, Vol II  M. Starr, H. Stolp, H.G. Truper, A. Balows, H.G. Schegel
(eds.) Springer-Verlag. Subsequent refs refer to this text by chapter
2)Chap.97,p.1196
3)Chap. 102,entire
4)Chap. 103, pp1287
5)Chap.103,p1293
6)Chap 104, pp. 1323
7)Chap 104,pp.1325
8)Gavini et. al., Intl. J. Syst. Bacteriol.(1989) 39(3);337-345
9)Beji et. al., Intl. J. Syst. Bacteriol.(1988) 38(1);77-88
10) Verdonck et. al.,  Intl. J. Syst. Bacteriol.(1987) 37(1);4-18




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