Ligation of PCR products

George W Chacko gchacko at magnus.acs.ohio-state.edu
Fri Jul 19 07:59:21 EST 1991


In article <9107182217.AA23884 at genbank.bio.net> ST403161 at BROWNVM.BROWN.EDU (Michael Newstein) writes:
>I have read that it is exceedingly difficult to blunt-end ligate products from
> PCR  (without cleaving first in internal restriction sites). I am attempting
>to clone the product of a PCR reaction into a blunt end cutter site (eg SMA 1)
>in pBluescript. Does anyone have suggestions?

You could try the TA cloning method. Invitrogen has a kit but it seems fairly
straighforward. There's a rapid communication in NAR 19:1154 by Marchuk,
Drumm,Saulino and Collins describing it.

George



More information about the Methods mailing list