5' cDNA sequence: Chirala method

FORSDYKE at QUCDN.QueensU.CA FORSDYKE at QUCDN.QueensU.CA
Thu Jul 4 16:54:14 EST 1991


Among various methods for obtaining the 5' cDNA sequence of a cDNA clone, the
method of Chirala, Kasturi, Pazirandeh, Stolow, Huang and Wakil (1989. J.Biol
Chem. 264, 3750-3757) is not widely recognized. Is anyone out there trying
the approach and have they any advice to others who are considering giving
it a try? I have written to the authors but have received no reply. With
permission, replies to this request will be summarized and posted here.
   Sincerely,  D. R. Forsdyke, Department of Biochemistry, Queen's University,
                               Kingston, Canada.



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