Transformation of Dam(+) DNA into a DAM(-) strain.
axa12 at po.CWRU.Edu
Tue Jul 30 22:23:58 EST 1991
As the subject indicates, my colleagues and I are having difficulty
transforming Dam(+) DNA (pBluescript with our insert) into a Dam(-)
strain. They have been using the traditional Calcium Chloride
method of transformation.
We would appreciate any suggestions in this regard. The Dam(-)
strain is GM161.
axa12 at po.cwru.edu
aiyar at cwbio.bioc.cwru.edu
More information about the Methods