Transformation of Dam(+) DNA into a DAM(-) strain.

Ashok Aiyar axa12 at po.CWRU.Edu
Tue Jul 30 22:23:58 EST 1991

As the subject indicates, my colleagues and I are having difficulty
transforming Dam(+) DNA (pBluescript with our insert) into a Dam(-)
strain.  They have been using the traditional Calcium Chloride
method of transformation.

We would appreciate any suggestions in this regard.  The Dam(-)
strain is GM161.

Thank you,

Ashok Aiyar
axa12 at
aiyar at

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