Ligation of PCR products

Michael Newstein ST403161 at BROWNVM.BROWN.EDU
Thu Jul 18 17:08:54 EST 1991

I have read that it is exceedingly difficult to blunt-end ligate products from
 PCR  (without cleaving first in internal restriction sites). I am attempting
to clone the product of a PCR reaction into a blunt end cutter site (eg SMA 1)
in pBluescript. Does anyone have suggestions?

More information about the Methods mailing list