Ligation of PCR products

Michael Newstein ST403161 at BROWNVM.BROWN.EDU
Thu Jul 18 17:08:54 EST 1991


I have read that it is exceedingly difficult to blunt-end ligate products from
 PCR  (without cleaving first in internal restriction sites). I am attempting
to clone the product of a PCR reaction into a blunt end cutter site (eg SMA 1)
in pBluescript. Does anyone have suggestions?



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