Messenger Affinity Paper
WHITSITT, MARK STEVEN
msw1633 at sigma.tamu.edu
Tue Jul 9 10:52:37 EST 1991
In article <0094B4BF.4A5AE080 at m44.unm.edu>, kim at m44.unm.edu writes...
>I have been looking into a method for Poly(A+) selection using a paper
>substrate linked to poly(U) called Hybond-mAP (AMERSHAM). I am told (by
>AMERSHAM technical support) that mAP has a small but loyal following, and I was
>wondering if anyone out there has used it. Is it as easy and effective as
> Thanks in advance for your comments.
Yes, I have used the Hybond-mAP paper. I liked it ok, but it has llimitations.
The biggest being that only small quantities can easily be isolated. It is also
kind of cost restrictive. I don't remember of have readily available numbers
regarding the yields, but I believe that Amersham's numbers are pretty accurate.
I required much larger quantities of Poly(A+) mRNA so I resorted to an oligo-dT-
cellulose batch method for isolation of the RNA.
Good luck. There are likely others out theree with better suggestions and
Mark S. Whitsitt, N5RJF Texas A&M University, Dept of Biochemistry
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