spectrum for EtBr-DNA

Michael Benedik bchs1b at JANE.UH.EDU
Tue Jul 9 09:53:23 EST 1991


I want to measure EtBr-DNA complexes quantitatively using a fluorimeter.
Does anyone know the appropriate wavelengths for excitation and emission?
I know the main excitation wavelength is around 300nm, but can you also
excite at a higher non-UV wavelength (even if not as efficiently)???

Thanks.


	----------------------------------------------------------

	 Michael Benedik
	 Department of Biochemical and Biophysical Sciences
	 University of Houston
	 INTERNET: Benedik at UH.EDU	BITNET: Benedik at UHOU
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