Ligation of PCR products

Donald A. Lehn donnel at helix.nih.gov
Fri Jul 19 09:40:20 EST 1991


In article <9107182217.AA23884 at genbank.bio.net> ST403161 at BROWNVM.BROWN.EDU (Michael Newstein) writes:
>I have read that it is exceedingly difficult to blunt-end ligate products from
> PCR  (without cleaving first in internal restriction sites). I am attempting
>to clone the product of a PCR reaction into a blunt end cutter site (eg SMA 1)
>in pBluescript. Does anyone have suggestions?



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