MURPHY at jii.afrc.ac.uk MURPHY at jii.afrc.ac.uk
Tue Jun 25 14:18:00 EST 1991

I heard recently of a method for detecting and quantifying RNA using short
complimentary oligos, labelled 5' with 32-P and with a nonsense 3' tail. After
hybridization the single-strand nonsense tail is digested with S1 nuclease and
the oligo gel-separated. Does anybody have a reference for this method, or
better still, a detailed protocol?
Thanks in advance.
George Murphy                                  |Snailmail: Molecular Genetics,
JANET:murphy at uk.ac.afrc.jii                    |           Cambridge Laboratory,
INTERNET:murphy%jii.afrc.ac.uk at cunyvm.cuny.edu |           JI Centre for Plant
EARN/BITNET:murphy%jii.afrc.ac.uk at UKACRL       |           Science Research,
                                               |           Colney Lane,
Telephone: +44 603 52571                       |           Norwich NR4 7UJ,
Fax:       +44 603 502270                      |           U.K.

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