quantitate plasmids

ALSOBROOK at yalemed.bitnet ALSOBROOK at yalemed.bitnet
Fri Jun 7 12:21:00 EST 1991


Well, if you're going to do a Southern, one way to quantitate the plasmid
is to use known quantities of the plasmid on the same blot. After hybrid-
ization, you can use the known plasmid signals as a calibration curve.
Even without a densitometer, this will give a fairly accurate estimate of
how many plasmid copies are present.



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