PCR Machines (Batch mail, and an offer you can't refuse)

harper at csc.fi harper at csc.fi
Wed Jun 5 08:44:29 EST 1991


In article <750 at ariel.ucs.unimelb.edu.au>, vivien at ariel.ucs.unimelb.edu.au (Vivien Bonazzi) writes:
> 
> 
> I wanted to make a few inquires about PCR machines that are available on the
> market at the moment. I'm thinking of buying a machine for our lab.

There has been quite alot written about PCR in the METHODS Bboard. 
The entries go back to 1990.   There is about 90 hits using LDBASE
and SEARCH PCR IN +METHODS. Here are the results. You can recover
the items you want to read about from LISTSERV by sending a small
batch file to LISTSERV at IRLEARN.UCD.IE

( I would just like to say that the technique of using LDBASE or 
  MAIL BATCH processing is VERY SIMPLE and fast. I got the results
  from LDBASE instantaneously and the batch mail was delivered within
  15 minutes. The facilities are available use them.)

Here is an example of a batch file to get items dealing with PCR/MACHINES
Just cut it out and post it to LISTSERV at IRLEARN.UCD.IE
Leave the subject line blank and in the main body of the mail include the
following:
----------------- 8< cut here 8<----------------
//methods JOB Echo=YES,Reply-via=MAIL
Database search DD=Mine
//Mine DD *
Search pcr in +methods
index
print 000260
print 000279
print 000266
print 000470
print 000481
print 000485
/*
---------------- 8< cut here 8<-----------------

I enclose the index of items so that if you wnat to ADD more PRINT
commands to the above batch job you can do so... perhaps there are other
things that you might be interested in.

*************************  CLIP  *********************
> INDEX
Item #   Date   Time  Recs   Subject                                            
------   ----   ----  ----   -------                                            
000012 90/01/26 07:53   20   PCR....help
000013 90/01/26 18:02   40   RE: PCR....help
000014 90/01/31 17:13   31   visualizing small pcr fragments
000015 90/02/01 10:54   18   Re: visualizing small pcr fragments
000016 90/02/01 09:33   36   Small PCR fragments.
000018 90/01/29 16:20   39   Re: PCR....help
000019 90/02/03 19:25   18   PCR * Enzymes cut ss-DNA?
000020 90/02/12 17:55   40   Re: visualizing small pcr fragments
000028 90/02/12 18:14   26   Re: visualizing small pcr fragments
000030 90/02/20 21:48   36   UV light kills DNA contamination in PCR samples
000032 90/02/21 11:22   12   Primers for PCR
000034 90/02/23 20:11   39   re: PCR...help
000061 90/04/11 11:25   21   PCR ON YEAST RNA
000064 90/04/19 23:32   29   Computer program for choosing oligonucleotides (qu+
000065 90/04/29 23:33   23   Looking for cheap Thermal Cyclers
000080 90/05/29 18:12   17
000085 90/05/30 13:55   65   Re: (none)
000099 90/06/19 10:25   17   PCR errors
000102 90/06/25 06:40   32   Re: PCR errors
000123 90/07/11 17:28   46
000124 90/07/11 14:55   21   LONG PCR FRAGMENTS
000134 90/07/21 08:18   16   PCR reagent software
000146 90/07/27 13:37   20   pcr primer dimer help
000151 90/07/31 16:41   33   Bacteria and PCR
000184 90/09/05 00:39   24   Southerns with seiving agarose
000186 90/09/06 14:24   20   melting algorithms
000191 90/09/12 14:39   48   Cloning PCR fragents into vector
000192 90/09/13 10:00   75   Re: Cloning PCR fragents into vector
000193 90/09/14 01:53   70   Re: Returned Network Mail
000197 90/09/18 12:14   25   melting algorithms
000203 90/09/27 02:27   81   Re: Oligo directed Insertions
000206 90/09/28 13:29   51   PCR Sequencing
000208 90/10/01 12:08   17   re:pcr sequencing
000210 90/10/02 09:38   60   RE: PCR sequencing
000213 90/10/02 12:51   25   re:PCR sequencing
000220 90/10/09 12:15   35   DNA ISOLATION
000229 90/10/04 17:16   28   VNTR sequencing
000242 90/10/23 13:53   21
000243 90/10/23 17:59   24   asymmetric PCR + sequencing
000244 90/10/24 19:36   26   RNA to cDNA then PCR
000248 90/10/29 21:30   23   Re: Oligonucleotide probing
000250 90/11/02 06:31   26   PCR Primer Selection Programs Required
000251 90/11/02 14:53   27   alu seqs and pcr
000252 90/11/02 15:54   19   PCR cloning
000254 90/10/28 12:41   38   Re: inverse PCR
000255 90/11/02 14:54   27   alu seqs and pcr
000256 90/11/05 09:27   16   Re: Oligonucleotide probing
000257 90/11/05 11:50   22   PCR CLONING
000258 90/11/05 17:54   21   re:PCR CLONING
000259 90/11/05 17:03   20
000260 90/11/08 13:49   29   PCR Machine
000266 90/11/10 01:58   45   Re: PCR Machine
000279 90/11/15 17:32   26   Re: PCR Machine
000288 90/11/26 23:39   19   about PCR problems
000289 90/11/26 23:15   25   Re: about PCR problems
000300 90/12/12 20:54   35   PCR Cloning - TA Cloning system
000302 90/12/13 14:04   33   PCR cloning
000314 91/01/08 23:20   24   Anyone done PCR directly from cells
000315 91/01/08 23:04   24   Re: Anyone done PCR directly from cells
000316 91/01/10 07:36   15   Re: Anyone done PCR directly from cells
000319 91/01/12 11:01   20   Re: Anyone done PCR directly from cells
000320 91/01/15 07:18   18   Re: Anyone done PCR directly from cells
000336 91/01/28 19:17   21   bacteria detection in plants
000338 91/01/31 04:44  184   seeking bacterial strains
000339 91/01/31 16:11   28   Direct PCR from Tissue Samples
000351 91/02/04 19:38   20   pcr on fixed samples
000359 91/02/18 16:14   57   message
000366 91/02/20 22:54  135   message
000391 91/03/06 13:41   16   PCR
000401 91/03/11 00:54   21   Help: DNA from Clostridium spores
000404 91/03/12 15:53   30   PCR fragment cloning into m13 vectors
000406 91/03/12 15:57   61   Re: PCR frag. cloning into M13 vectors
000407 91/03/12 13:39   33   Re: PCR fragment cloning into M13 vectors
000416 91/03/15 17:30   47   Re: PCR fragment cloning into m13 vectors
000418 91/03/15 17:58   50   Re: PCR fragment cloning into m13 vectors
000433 91/03/30 21:34   74   Why does RAPD mapping work?
000439 91/04/06 02:55  116   Re: Why does RAPD mapping work?
000449 91/04/12 17:24  352   Re: Why does RAPD mapping work? SUMMARY
000456 91/04/23 18:15   40   PCR primer sequence collection
000459 91/04/26 18:30   22   Genomic substraction
000461 91/04/29 09:49   36   Reverse Transcription and PCR
000464 91/04/29 19:40   99   Re: Genomic substraction (fwd)
000470 91/05/03 18:37   20   Unreliability of PCR machines in time
000471 91/05/03 20:32   12   PCR
000472 91/05/06 16:59   28
000481 91/05/14 17:27   14   pcr MACHINE
000482 91/05/15 09:38   21   Facilities-Primers Design?
000483 91/05/14 19:31   44   PCR Machine-MJ Research
000485 91/05/15 10:16   16   RE: pcr MACHINE
000506 91/05/29 18:20  170   summary of *oligo construction programs*
000508 91/05/30 16:29   23   PCR references?
******************   END  ************************

Rob "I'd rather see a sermon than hear one" Harper

P.S. This months special offer:

I will do LDBASE searches for anyone who wants to recover old messages
from LISTSERV and provide them with an INDEX of entries... but they
with have to finish the job off themselves and do the actual recovery
by BATCH MAIL. Looking forward to hearing from you.



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