automated DNA sequencing..MRC programs

Bruce Roe BROE at AARDVARK.UCS.UOKNOR.EDU
Sat Jun 22 08:45:00 EST 1991


Hi,
	Several individuals have requested info regarding the MRC programs
I mentioned in my recent posting in reference to Automated DNA Sequencing
Instruments.
	Briefly, these programs take the ABI or Pharmacia analized data
( sequences and graphics ) and place them in a form which can be entered
into the Staden Sequence Alignment Program (SAP) [the unix version is XDAP].
	Once a contig is formed it can be edited by viewing the ABI graphic 
output and proof reading directly on the SPARCstation.  Over the past 10 
years, Staden, et al have developed a series of programs for sequence
alignment and analysis for the VAX.  They now have ported these to the 
SPARCstation and added a sequence editor and a X-windows graphic display
program which can deal with the output from both ABI and Pharmacia
fluorescent sequencing instruments.

	Over the past 10 years, Staden has published many papers describing
is programs which are available for the VAX/VMS and his recent port to the 
SUN UNIX O/S and the fluorescent sequencing instrument data analysis programs
will be described in an upcoming Nucleic Acids Research publication.

	Staden's e-mail address is:

 	RS at MRC-LMB.CAM.AC.UK

but be warned, he sometimes is very slow in replying to e-mail.  
You also may wish to contact Rick Wilson at Washington Univ., St. Louis, Mo.
at:

	rick at geneman.wustl.edu

Rick and the group at Wash.U are sequencing the C. elegans genome
in a collaboration with the MRC group and he'll know the latest on the
MRC programs since these programs also were developed as part of this
collaboration.

Cheers and hope this is helpful to you'all
--bruce
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