Analysis of plasmid after transfection?

BIOCHEMISTRY klautky at vaxa.weeg.uiowa.edu
Thu Jun 6 09:13:58 EST 1991


Dear Net Readers,

    Does anyone know of a protocol (or direct me to a reference) for the
analysis of plasmid DNA after transfection?

    I am interested in a method(s) that can quantitate the plasmid transfected
and determine intactness with respect to supercoiling.  Obviously, a
Southern can be used to check the latter but it is the former that I am
unsure of.

                Thanks in advance,                 Stephen Klautky



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