E.coli host cells (rec deficient)
Robert H Woodman
bwoodman at magnus.acs.ohio-state.edu
Thu Jun 13 11:55:45 EST 1991
In article <1991Jun12.192034.6348 at lynx.CS.ORST.EDU> you write:
>We are looking for an E. coli hostcell which prevents recombination
>events in plasmids containing many repeats of the same gene. We tried
>several recA- strains, but recombination still occured. Any suggestions
>of potentially useful hostcells would be appreciated.
>Please e-mail your help to
>demelerb at bionette.cgrb.orst.edu
>Thank you very much, -Borries
Borries,
Did you try DH5alpha and DH5alpha MCR from BRL? Did you try GM48
from IBI? If you tried those and still got recombination, then I can't
help you.
Bob Woodman
--
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*Robert H. "Bob" Woodman, PhD * "A job not worth doing well is not *
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