E.coli host cells (rec deficient)

Robert H Woodman bwoodman at magnus.acs.ohio-state.edu
Thu Jun 13 11:55:45 EST 1991


In article <1991Jun12.192034.6348 at lynx.CS.ORST.EDU> you write:
>We are looking for an E. coli hostcell which prevents recombination
>events in plasmids containing many repeats of the same gene. We tried
>several recA- strains, but recombination still occured. Any suggestions
>of potentially useful hostcells would be appreciated.
>Please e-mail your help to 
>demelerb at bionette.cgrb.orst.edu

>Thank you very much, -Borries

Borries,

	Did you try DH5alpha and DH5alpha MCR from BRL?  Did you try GM48
from IBI?  If you tried those and still got recombination, then I can't
help you. 

Bob Woodman


-- 
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*Robert H. "Bob" Woodman, PhD * "A job not worth doing well is not  *
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