E.coli host cells (rec deficient)

Andreas Pahl macbeth at opal.cs.tu-berlin.de
Mon Jun 17 18:17:01 EST 1991


In article <1991Jun12.192034.6348 at lynx.CS.ORST.EDU> you write:
> We are looking for an E. coli hostcell which prevents recombination
> events in plasmids containing many repeats of the same gene. We tried
> several recA- strains, but recombination still occured. Any suggestions
> of potentially useful hostcells would be appreciated.
> Please e-mail your help to 
> demelerb at bionette.cgrb.orst.edu
> 
> Thank you very much, -Borries

--

Hi,

did you try Stratagenes E.coli SURE (Stop unwanted Rearrangement events or
something like this written). The cells grow rather slowly, but you might
try this one.

Tschau, Andreas

P.S. I suppose you tried normal strains like DH5alpha or so.

 
###############################################################################
Andreas Pahl                                       macbeth at opal.cs.tu-berlin.de
Institut f. Biochemie u. Molekulare Biologie   
TU Berlin
Franklinstr. 29
D-1000 Berlin 10
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