mwsmith at UCSD.EDU
Wed Mar 13 19:43:14 EST 1991
Has anyone tried the Strataclean resin from Stratagene? Today I used
this resin in a alkaline lysis miniprep method. Basically the resin is used
to bind up protein from the cellular extract instead of a phenol chloroform
extraction. Stratagene claims that the miniprep DNA is suitable for digestions
and sequencing. The miniprep DNA did cut with SstI, but I wonder if the
background RNA will interfere with sequencing.
*****I have several questions for the BB: *****
1) Has DNA purified using this resin worked for sequencing in anyones hands??
2) Is Stratagene the only source? The resin is priced at $0.55-0.77/miniprep.
Seems like a steep price for a reagent that they probably can make
by the bucketful, and later sell in 3ml and 9ml packages.
I have no financial connection to the manufacturer. Just looking for a
less toxic approach to isolating DNA.
---------------- Michael W. Smith ----------------
| Center for Molecular Genetics |
| 9500 Gilman Drive |
| University of California, San Diego |
| La Jolla, CA 92093-0634 |
-------------- mwsmith at ucsd.bitnet ---------------
This is the size BB message I like to get. Its short, sweet, and fits on a page
More information about the Methods