bourson at jouy.inra.fr
Thu Mar 28 09:33:02 EST 1991
I generaly use the methods of RNAse protection recomanded in :
Sambrook, Fritch et Maniatis "Molecular cloning" 2nd edition
1989 Cold Spring Harbor Laboratory Press (chapter 7-8)
Ausubel F.M. et al Current Protocol in Molecular Biology
1987-88 Greene Publishing Associated and Wiley-Interscience New-York
The methods can be resumed in :
always wear gloves and never use non-treated material.
all the solutions must be treated by 0.2% DEPC (Diethyl pyrocarbonate)
and then autoclaved.(Tris is precipited by DEPC treatment)
glassware is sterilised by backing at 160!C for several hours.
single-use sterilised plasticware is very useful to avoid RNAse problems.
plastic and glass ware can be by traeted with 3% H2O2, or chloroform.
you can also use RNAse inhibitor to increase the level of RNAse
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