Vertical agarose electrophoresis
William R. Pearson
wrp at biochsn.acc.Virginia.EDU
Mon Mar 11 13:37:04 EST 1991
In article <9103100855.AA04653 at genbank.bio.net> EBMV36 at ercvax.edinburgh.ac.uk writes:
>Is there anyone who has the experience of doing genomic southern hybridization
>using vertical electrophoresis? According to the adv. of Stratagene, they
>claimed the vertical electrophoresis gives better resolution and saves the
>running time. I am wondering why there is just only one model of commercial
>electrophoresis apparatus available if the vertical agarose electrophoresis
>has such wonderful magics.
>Any comment and discussion would be appreciated.
>Dept. of Molecular Biology
>Univ. of Edinburgh
>EBMV36 at ercvax.edinburgh.ac.uk
I am a great fan of vertical gel electrophoresis for Northern
and Southern blots. I believe that it provides a quality and reproducibility
that is difficult to match with horizontal apparatus. We use gel boxes
made be Watson Products, PO Box 247, (818) 797-5773. They are a "mom &
pop" company that started making vertical gel apparatus just as agarose
was being developed for DNA fractionation. They sell frosted (sand-blasted)
plates, which are essential for holding low-percentage gels in position.
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