staining northern blots

ian at mrcvax.ed.ac.uk ian at mrcvax.ed.ac.uk
Thu Mar 28 12:16:37 EST 1991


We use ethidium bromide very successfully to stain formaldehyde RNA gels.

Add about 1 ul of a 1mg/ml solution to 15ul sample before the 55 degree
 incubation. The ethidium binds to the RNA, so that on electrophoresis the RNA
 is stained but excess unbound ethidium runs the opposite way, towards the
 negative electrode. To be

The result is nicely stained RNA with very low background. The RNA blots and
 hybridises fine.

Ian J. Jackson
MRC Human Genetics Unit
Edinburgh,
Scotland



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