Automated methods for bacterial classification
USERMERL%SFU at PUCC.PRINCETON.EDU
Wed May 15 09:16:24 EST 1991
HI. I hope someone out there can help me or at least voice an opinion
I wish to classify the normal bacteria in the gut of salmon. The bacteria
tend to grow around 10 - 20 degrees celcius. I have considered using an
automated system (namely API 20E) to partially classify the flora, BUT am
faced with the following problems:
1) This system only deals with gram negatives doesn't it? Is there a
database of numbers for species NOT in the API book?
2) The system is standardized for incubation at 37 degrees celcius. Do
you think that incubating at a lower temperature would change the results?
Do you have any opinion on how long to incubate?
3) It seems to me that the organisms I am most likely to encounter are not
even listed in the API book, and I am worried about obtaining false i.d.'s.
I am beginning to think that my only choice is to obtain standard cultures
of the organisms I am most likely to encounter and to use the results of
these (perhaps using numerical taxonomy) to classify my unknowns. Any
Thank you in advance,
Dorothy Miyake bitnet: usermerl at cc.sfu.ca
Dept. Biological Sciences
Simon Fraser University
Burnaby, B.C., Canada
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