Electroporation efficiency in E. coli

micprf at lure.latrobe.edu.au micprf at lure.latrobe.edu.au
Thu May 23 03:14:15 EST 1991

We recently bought an electroporator and have been doing some experiments
to optimize it for our standard E. coli host strains. At present we are
getting about 2 x 10^7 per microgram for HB101 and 10 times that with
MC1061. These numbers apply to two different vectors - a 6.1 kB Dictyostelium
shuttle vector and the Bluescript vector. These frequencies are well short
of the frequencies one is supposed to be able to achieve. My question is -
what are the real frequencies that most people achieve with cells they have
prepared themselves?			Cheers, Paul Fisher.
						(micprf at lure.latrobe.edu.au)

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