A question about RNA and skin!

BOROS at OHSTMVSA.BITNET BOROS at OHSTMVSA.BITNET
Tue Nov 26 11:24:00 EST 1991


Hi there!
We are interested in analysing mRNA in skin, but
since the epidermis has high RNAse activity, we got RNA degradation
after lysing the keratinocytes in the medium.
Does anyone know an agent (inhibitor) inhibiting RNAse activity
during cell (either kind) exploration or have experience in
isolating RNA from epidermal tissue samples?
Maybe we could use the method for skin and
post back our results and experience with epidermis slices upon interest.
Every little help is magic and very much appreciated.
Thanks, Laszlo G. Boros, MD
        The Ohio State University
        Columbus, OH
e-mail: boros at ohstmvsa.acs.ohio-state.edu



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