Thermostable polymerases without...
rybicki at uctvax.uct.ac.za
rybicki at uctvax.uct.ac.za
Thu Nov 28 10:07:04 EST 1991
In article <9111220629.AA17326 at nuscc.nus.sg>, bchtantw at NUSCC.NUS.SG (Dr Tan Tin Wee) writes:
> Dear all,
>
> Could anyone recommend me thermostable DNA polymerases for PCR that
> do not have DNA-template-independent polymerase activity?
> I understand that Taq polymerase puts in A's preferentially at the
> 3'ends of the PCR product (which is the basis of the TA cloning technique).
> Addresses of the vendors would be much appreciated.
> Thank you in advance.
>
> Tan Tin Wee
> bchtantw at nuscc.nus.sg
> bchtantw at nusvm.nus.sg
> bchtantw at nusvm.bitnet
> Dept of Biochemistry, Natl Univ. of Singapore.
Don't all polymerases without copy-editing/proof-reading ability do this?
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