nylon membrane

YHSUN at imb.as.tw YHSUN at imb.as.tw
Thu Nov 14 09:58:00 EST 1991

I would like to hear comments on (1) comparison on charged nylon vs. non-
charged nylon membrane, (2) What are the critical factors of the various
manufacturer's conditions for blotting, hybridization, and washing? Are
they really critical? Or the standard method works? (3) Has anyone used 1M
ammonium acetate for blotting with nylon membranes?

Y. Henry Sun
Institute of Molecular Biology
Academia Sinica

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