PCR Primers

nigel at codon3.berkeley.edu nigel at codon3.berkeley.edu
Tue Nov 19 01:58:15 EST 1991


In article <1991Nov18.221845.2065 at ac.dal.ca> nbr at ac.dal.ca writes:
>In article <1991Nov18.132635.311 at ucbeh.san.uc.edu>, penas at ucbeh.san.uc.edu writes:

-> Hello!
-> I have yet another question about PCR.  What is the minimum GC% that a PCR
-> primer should have.  What is the maximum and minimum effective size for a PCR
-> primer.  Thanyou very much, in advance, for your response.
-> 
-> -- 
-> Sandra Pena de Ortiz     : penas at ucbeh.bitnet
-> University of Cincinnati
-> College of Medicine
-> Toxicology Program
> the 3' ends), this may say something about the minimum length required in the initial PCR cycles.
>
>Bruce Ramsey
>Marine Gene Probe Lab
>Dalhousie University
>Halifax N.S. Canada
>
>Disclaimer:  "THIS END UP"

Bruce, no matter which end of this posting I hold skywards, it still seems to be
missing something. Could you please repost? 

thanx

Nigel   



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