Summary: Double Stranded DNA Sequencing

penas at ucbeh.san.uc.edu penas at ucbeh.san.uc.edu
Tue Nov 5 23:00:32 EST 1991


I want to thank all of those who sent me information on double stranded DNA
sequencing with sequenase.  I have gotten my sequencing reactions to work
beatifully.  I found the most critical aspect to be the cleanliness of my
template.  I started cleaning my minipreps with Prep-A-Gene and found that that
made a big difference.  Also, I am now denaturing my DNA with fresh NaOH/EDTA. 
Thanks again for your help.

Sincerely,
  
--
Sandra Pena de Ortiz     : penas at ucbeh.bitnet
University of Cincinnati
College of Medicine
Toxicology Program



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