Sequencing PCR products

Jeff levinthal at aclcb.purdue.edu
Tue Nov 5 11:03:33 EST 1991


Hi there Netters!
I am interested in sequencing a regulatory region of a gene from a number 
of related E. coli strains (from the ECOR collection).  We have been able 
to amplify this region succesfully so my main question is this: Does anyone 
know of any lower limit to the length of the PCR product to be sequenced? 
Ultimately I would like to use the same primers and be able to sequence 
the strands one one gel (so about 3-400 bases).
Any thoughts on this and other tips on sequencing PCR products would be 
greatly appreciated!
Thanks in advance!
Jeff
    Name: Jeff
Internet: levinthal at aclcb.purdue.edu
   Phone: 317-463-4515



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