formamide/ membranes for hybridizations.

Andreas Pahl macbeth at cs.tu-berlin.de
Thu Nov 7 14:04:25 EST 1991


In article <5NOV199123042856 at rigel.tamu.edu>, msw1633 at rigel.tamu.edu (Mark S. Whitsitt, N5RJF) writes:
> True, Nitrocellulose is a pain to use if you are using a regular NC filter. I 
> have been using S+S BAC filters for screening of plaque lifts and have had much 
> luck with it.  It is nitrocellulose that is bound to a synthetic (nylon?) 
> support.  It does not exhibit the same fragile character of normal NC filters.
> 
> You will have to bake it, but that is no problem with the supported NC.  Also, 
> you should probably check me on the name of the stuff, as I know it is from 
> Schleicher and Schuell, but the item name almost escapes me.

It is the BAS-membrane from S+S.

Who do the people bake their NC-filters instead of UV-Crosslinking?
Two minutes, that is all you need.


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Andreas Pahl                                email: macbeth at opal.cs.tu-berlin.de
Institut f. Biochemie u. Molekulare Biologie       macbeth%opal at DB0TUI11.BITNET
TU Berlin                                          macbeth%opal at DB0TUI11.EARN
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