Thermostable polymerases without template-indept activity

Dr Tan Tin Wee bchtantw at NUSCC.NUS.SG
Fri Nov 22 01:29:50 EST 1991


Dear all,

Could anyone recommend me thermostable DNA polymerases for PCR that
do not have DNA-template-independent polymerase activity?
I understand that Taq polymerase puts in A's preferentially at the
3'ends of the PCR product (which is the basis of the TA cloning technique).
Addresses of the vendors would be much appreciated.
Thank you in advance.

Tan Tin Wee
bchtantw at nuscc.nus.sg
bchtantw at nusvm.nus.sg
bchtantw at nusvm.bitnet
Dept of Biochemistry, Natl Univ. of Singapore.




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