(none)

MURPHY at JII.AFRC.AC.UK MURPHY at JII.AFRC.AC.UK
Fri Nov 15 07:52:00 EST 1991


I am doing some work on hydroxyproline-rich proteins and need to be able to
estimate the amount of hydroxyproline they contain. The method I have involves
digestion in conc. HCl followed by treatment with other nasty reagents such as
perchloric acid and dimethylaminobenzaldehyde. Does anybody out there know of
a less hazardous method? Also: is there a method for detecting hydroxyproline
proteins on acrylamide gels?
Cheers,
George Murphy
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George Murphy                                  |Snailmail: Molecular Genetics,
JANET:murphy at uk.ac.afrc.jii                    |           Cambridge Laboratory,
INTERNET:murphy%jii.afrc.ac.uk at cunyvm.cuny.edu |           JI Centre for Plant
EARN/BITNET:murphy%jii.afrc.ac.uk at UKACRL       |           Science Research,
                                               |           Colney Lane,
Telephone: +44 603 52571                       |           Norwich NR4 7UJ,
Fax:       +44 603 502270                      |           U.K.
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