PCR Primers

nbr at ac.dal.ca nbr at ac.dal.ca
Mon Nov 18 21:18:45 EST 1991


In article <1991Nov18.132635.311 at ucbeh.san.uc.edu>, penas at ucbeh.san.uc.edu writes:
> 
> Hello!
> I have yet another question about PCR.  What is the minimum GC% that a PCR
> primer should have.  What is the maximum and minimum effective size for a PCR
> primer.  Thanyou very much, in advance, for your response.
> 
> -- 
> Sandra Pena de Ortiz     : penas at ucbeh.bitnet
> University of Cincinnati
> College of Medicine
> Toxicology Program
 the 3' ends), this may say something about the minimum length required in the initial PCR cycles.

Bruce Ramsey
Marine Gene Probe Lab
Dalhousie University
Halifax N.S. Canada

Disclaimer:  "THIS END UP"



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