PCR Primers

nbr at ac.dal.ca nbr at ac.dal.ca
Mon Nov 18 21:30:06 EST 1991


Please forgive the previous post - system error?

I was writing to state that in at least two cases in our
lab, the downstream end of a primer may give unwanted 
priming from vector sequences.  In our case it was seven
and ten nucleotides in the 3' end of a 17-mer and a 20-mer,
respectively, which were sufficiently matched to lambda
to give a PCR product of (unfortunately for me) the
expected size.

The moral? Check particularly the 3' ends of your oligos
against your vector sequences.

Bruce Ramsey
Marine Gene Probe Lab



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