DNA cleanup

WMELCHIOR at NTDOC.NCTNET.GOV WMELCHIOR at NTDOC.NCTNET.GOV
Thu Oct 3 10:01:00 EST 1991


Chris Upton wrote about using glass milk to prepare DNA for sequencing.

Chris:

1)  How do you make your own glass milk?

2)  I tried Geneclean (Glass milk) on a gel (once), followed by doublestrand 
    sequencing, and got numerous bands in all lanes that I have tentatively 
    attributed to breaks in the plasmid DNA caused by the glass milk.  Please 
    let us know how your templates work.


Bill Melchior
National Center for Toxicological Research
Jefferson, AR  72079
(501) 543-7206

wmelchior at ntdoc.nctnet.gov



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