restriction enzymes that nick hemi-methylated DNA\

Andrew Mendelsohn mendelsohn at
Wed Oct 23 18:53:00 EST 1991

Dear colleagues,
     As part of a directional PCR strategy I am working on, I was 
wondering if it was known what enzymes cut the unmethylated strand of 
hemimethylated DNA.  Having checked the literature I have found that 
both Hae III and Msp I are reported to do this.  Any others?
Any help would be appreciated.
andy mendelsohn
dept of molecular biology
mass general hospital

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