restriction enzymes that nick hemi-methylated DNA\
mendelsohn at frodo.mgh.harvard.edu
Wed Oct 23 18:53:00 EST 1991
As part of a directional PCR strategy I am working on, I was
wondering if it was known what enzymes cut the unmethylated strand of
hemimethylated DNA. Having checked the literature I have found that
both Hae III and Msp I are reported to do this. Any others?
Any help would be appreciated.
dept of molecular biology
mass general hospital
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