oligo-dT selection.

BACKD at QUCDN.QueensU.CA BACKD at QUCDN.QueensU.CA
Wed Oct 9 07:18:27 EST 1991


Mike,
     You have to "de-fine" the cellulose prior to using the column. The
cellulose fines clog the column. To do this: suspend the cellulose in water
and allow it to settle (~5 -10 min), remove and discard the supernatant
containing the fines. Repeat two or three more times. The column should run
very well after treatment.
Don Back
Department of Biochemistry
Queen's University
Kingston, Ontario
CANADA



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