Poly(A)+ selction

WHITSITT, MARK STEVEN msw1633 at summa.tamu.edu
Thu Oct 24 20:44:54 EST 1991

In article <1991Oct23.144144.29391 at ccu.umanitoba.ca>, frist at ccu.umanitoba.ca writes...
>mRNA complexes with rRNA and these aggregates may need to be broken before
>oligo dT chromatography will do any good in enriching for the polyA+
>fraction. See
>Banile et al. (1976) Analytical Biochem.  72:413-427.

In his method (deleted above) Daniel states that he heats the RNA sample to 65
degrees C.  This is sufficient for breaking such complexes and taking care of 
any other secondary structures.  At least to the extent needed to obtain the 
quality mRNA he requires.

Mark S. Whitsitt, N5RJF        Texas A&M University, Dept of Biochemistry
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