HELP with PCR cloning

Hirdeypal S. Bhathal hsb at
Wed Oct 16 14:58:53 EST 1991

I tried to subclone RACE PCR products into Hinc II site of pUC-19 after
blunt ending them with T4 polymerase. On gel, it seems that the ligation worked 
but I never got a white colony. I am planning to do PCR with primer having
Eco RI site. I'll appericiate if anyone can give some suggestion.

hsb at

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