C. S. Enderlin
cenderli at isis.cs.du.edu
Sat Oct 5 13:47:22 EST 1991
In article <1991Oct3.141522.18804 at ccu.umanitoba.ca> frist at ccu.umanitoba.ca writes:
>Another way of getting rid of RNA in plasmid preps is RNAseA digestion,
>followed by PEG precipitation. After the initial alkali lysis and ensuing
>steps, resuspend your EtOH pellet in 25mm Tris Cl pH8.0 30mM EDTA.
>Digest with RNAseA [10ug/ml] at 37C, 15min. Add 0.4 vol. of 30% PEG6000,
>1.8M NaCl (note: use MCB or EM PEG6000, not Sigma.)
Is the Sigma PEG problem peculiar to double strand sequencing?
I have used Sigma PEG for ssDNA preps ad nauseam with great results.
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