T4 kinase and S-35 labelled ATP.
Donald A. Lehn
donnel at helix.nih.gov
Tue Oct 29 10:51:51 EST 1991
In article <1991Oct28.222029.1255 at nrcnet0.nrc.ca> num208jn at MBDS.NRC.CA writes:
/>In article <1991Oct28.165146.1 at kean.ucs.mun.ca>, roger at kean.ucs.mun.ca writes:
/>> Can 35S (sulphur-35) gamma-labelled ATP be used to
/>>phosphorylate oligonucleotides with T4 polynucleotide kinase??
/>>Roger C. Green, Faculty of Medicine Phone: (709)737-6884
/>>Memorial University , St. John's, Newfoundland. FAX : (709)737-7010
/>If you find out, could you please post this, as I was just about to
/>post the same question.
Perhaps what everyone should do is contact NEN to make their P-33 nucleotides
commercially avaliable. These could be used in all labeling systems and would
provide all of the benefits of S-35 (long half-life / lower energy emmision)
without having to worry about the fact that it is S and not P.
Donald A. Lehn, Ph.D. Phone: (301) 496-2885
Bldg.37 Rm 3D20 FAX: (301) 496-8419
National Cancer Institute / NIH Email: donnel at helix.nih.gov
Bethesda MD 20892
More information about the Methods