T4 kinase and S-35 labelled ATP.

Donald A. Lehn donnel at helix.nih.gov
Tue Oct 29 10:51:51 EST 1991


In article <1991Oct28.222029.1255 at nrcnet0.nrc.ca> num208jn at MBDS.NRC.CA writes:
/>In article <1991Oct28.165146.1 at kean.ucs.mun.ca>, roger at kean.ucs.mun.ca writes:
/>>	Can 35S (sulphur-35) gamma-labelled ATP be used to 
/>>phosphorylate oligonucleotides with T4 polynucleotide kinase??
/>>
/>>
/>>Roger C. Green,	Faculty of Medicine                      Phone: (709)737-6884
/>>Memorial University , St. John's, Newfoundland.          FAX  : (709)737-7010
/>>
/>
/>If you find out, could you please post this, as I was just about to
/>post the same question.
/>

Perhaps what everyone should do is contact NEN to make their P-33 nucleotides
commercially avaliable.  These could be used in all labeling systems and would
provide all of the benefits of S-35 (long half-life / lower energy emmision) 
without having to worry about the fact that it is S and not P.


--
Donald A. Lehn, Ph.D.                      Phone: (301) 496-2885
Bldg.37 Rm 3D20                            FAX:   (301) 496-8419
National Cancer Institute / NIH            Email: donnel at helix.nih.gov
Bethesda MD 20892



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