glyoxalation

kim at m44.unm.edu kim at m44.unm.edu
Tue Sep 17 16:04:17 EST 1991


Hi:
Sorry about the extra posting.
I am running RNA in agarose gels after denaturation using glyoxal/DMSO.  As I
understand it, glyoxal forms adducts onto "G's", preventing hybridization. 
These adducts can be removed by alkaline (pH >8) conditions.  

Would it be possible to ethanol precipitate glyoxalated DNA or RNA and preserve
the glyoxalation?  Send replies to:  KIM at FLOVAX.LANL.GOV    thanks.
Daniel Kim



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