Removal of CsCl from CsCl-EtBr gradient preps

murianap muriana at aclcb.purdue.edu
Mon Sep 9 06:51:55 EST 1991


I am posting to inquire if anyone can give me a easy way of getting rid of
the CsCl remaining in EtBr-extracted CsCl chromosomal preps?  I have 
routinely used Amicon Centricons for plasmid preps, but believe these will 
get clogged if I use them for chromosomal preps.  I've tried the method in 
the new Maniatis (add 3 vol d.H20 + 2 vol EtOH) but that didn't work.  Is 
there any other method besides dialysis?

Thanks in advance,
Peter M. Muriana
    Name: murianap
Internet: muriana at aclcb.purdue.edu
   Phone: 317-494-8284



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