Histidine tags in eukaryotic expression vectors

'' MBLRA at rose.uib.no
Mon Sep 23 04:52:17 EST 1991


Does anyone out there have any experience with the use of Histidine
tags in eukaryotic expression vectors for purification on recombinant
proteins on Nickel-ion affinity coloums?
 
I'm interested in which vectors have been used, where the His-tag
may be situated and papers describing such use of His-tags. 
Are there any peculiarities to the use of His-tags in eukaryotic
vectors as compared to E- coli vectors?

Rein Aasland -*

If you respond by mail to me, please use: aasland at bio.uib.no

                                                    September 23. 1991
----------------------------------------------------------------------
Rein Aasland                        | Tlf.: +47-5-544516
Center of Biotechnology             | Fax.: +47-5-314952
University of Bergen                | E-mail: aasland at bio.uib.no <<<!
HiB                                 |  
N-5020 Bergen                       |---------------------------------
Norway                              | Street adress: Thormohlens gt 55
----------------------------------------------------------------------



More information about the Methods mailing list