Histidine tags in eukaryotic expression vectors
''
MBLRA at rose.uib.no
Mon Sep 23 04:52:17 EST 1991
Does anyone out there have any experience with the use of Histidine
tags in eukaryotic expression vectors for purification on recombinant
proteins on Nickel-ion affinity coloums?
I'm interested in which vectors have been used, where the His-tag
may be situated and papers describing such use of His-tags.
Are there any peculiarities to the use of His-tags in eukaryotic
vectors as compared to E- coli vectors?
Rein Aasland -*
If you respond by mail to me, please use: aasland at bio.uib.no
September 23. 1991
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Rein Aasland | Tlf.: +47-5-544516
Center of Biotechnology | Fax.: +47-5-314952
University of Bergen | E-mail: aasland at bio.uib.no <<<!
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