oligomer cleavage

Bruce Byrne byrne at soma.umdnj.edu
Wed Sep 18 07:21:15 EST 1991


suter at vax.mpiz-koeln.mpg.dbp.de writes:


>dear colleagues,

>the biolabs catalogue has a nice table entitled:

>CLEAVAGE CLOSE TO THE END OF DAN FRAGMENTS (page 132 1991 catalogue.

>Listed are the minimal sizes of dsDNA fragments that still can be cut
>by f.i. BamHI etc. Do any of you have such a list which is even more
>complete ?
r

>thanx,

>clemen

    The issue was addressed by Kaufman and Evans in BioFeedback 9 (3):
304-306, 1990.  In this work the authors double digested Bluescript II,
endlabeling the linearized molecules between the two digestions and 
looking at the ability of the second enzyme to liberate label.  In an
erratum published in a later issue the authors clarify their table
by the following: "'Base Pairs from Terminus' refers to the number of
double-stranded bases from the end of the second endonuclease recog-
nition sequence to the terminus."

BCB



More information about the Methods mailing list