Loss of polylinker sites

HRMBDKC at hkumd1.hku.hk HRMBDKC at hkumd1.hku.hk
Wed Sep 18 20:30:00 EST 1991

We have recently found that after cloning PCR products into the SmaI
site of bluescript some or most of the linker sites have been lost.
has anyone else had this experience and is there a reason? We
do blunt end our PCR products before subcloning.
Kathy Cheah

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