ssDNA preparations from M13KO7

kim at m44.unm.edu kim at m44.unm.edu
Thu Apr 30 14:58:21 EST 1992


In article <1992Apr30.084720.11783 at uniwa.uwa.edu.au>, robert at arbo.microbiol.uwa.oz.au (Robert Coelen) writes:
>What is your favourite, rapid, but good ! method for getting nice ssDNA
>preps from M13 phage infections for the purpose of sequencing. 

                         <Stuff deleted>
I've never tried it, but there is a method using glacial acetic acid, sodium
perchlorate, and glass (either a filter or glass powder).  This was posted here
a while ago.  One reference to this method is found in Nucleic Acids Research.
15:5507-5516 (1987).  Some company (Promega?  BRL?) makes a kit to do this as
well.

Daniel Kim



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