ssDNA preparations from M13KO7

Robert Coelen robert at arbo.microbiol.uwa.oz.au
Thu Apr 30 03:47:20 EST 1992


What is your favourite, rapid, but good ! method for getting nice ssDNA
preps from M13 phage infections for the purpose of sequencing. We're
reasonably happy with our phenol/chloroform method, but may be some
no phenol/chloroform method is just as good (or better ?) I guess this
sounds like a bit of an opinion poll, but before I buy something else
I'd like to find out what the concensus is (if you think phenol/
chloroform was better let me know too !)

Thanks,


Dr Robert J. Coelen
Dept of Microbiology
The University of Western Australia
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MAY THE LIGASE BE WITH YOU



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