Mutagenesis using straight PCR?

mcgraw%gandal.dnet at SERVER.UGA.EDU mcgraw%gandal.dnet at SERVER.UGA.EDU
Sat Apr 18 18:08:38 EST 1992


Hi Tao,

I'm sure I saw a paper on precisely this subject.  Seems like it was NAR.
I can't recall exactly how long ago, or who the authors were.  I could
guess about the authors, but I don't want to on the net. If I remember
correctly, they used different divalent ions like Mn and so forth to cause
Taq polymerase to make mistakes.  You can also use skewed dNTP ratios in the
mix to cause errors, I think.

					Al




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