PCR Mutagenesis

Ernest Retzel 1535 49118 ernest at lenti.med.umn.edu
Sat Apr 18 21:26:15 EST 1992

Regarding PCR mutagenesis, I personally wouldn't want to do it [I don't think
the frequency would be high enough], but if I were to try, I think I would
use a dramatically lower elongation temperature [37-42C].  It still synthesizes
there, it just doesn't do it very well.

Ernie Retzel
Dept. of Microbiology
Univ. of Minnesota

ernest at lenti.med.umn.edu

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